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We initiated an evolution campaign with seven PANCE passages on GD_M, followed by 12 passages of high-stringency dual selection penalizing proteases that cleave VAMP1, and concluded with 16 passages on a circuit using a weaker promoter in front of the GD_M substrate to increase positive selection stringency (Extended Data Fig
It provides a convenient format for researchers investigating intranasal peptide administration and delivery models
Conclusions MASLD is one of the pressing public health issues
Experience with teduglutide treatment for short bowel syndrome in clinical practice
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